Ultracentrifugation
- Gold standard method
- Step-by-step protocol
- Typical yield: 1-10μg exosomal protein/mL plasma
Research Methods & Protocols
Exosome Isolation Techniques
Polymer-Based Precipitation
- Commercial kits (ExoQuick, Total Exosome Isolation)
- Faster but lower purity
- Ideal for small sample volumes
RNA Analysis Workflow
graph LR
A[Exosome Isolation] --> B[RNA Extraction]
B --> C[Quality Control]
C --> D[Library Prep]
D --> E[Sequencing]